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human kidney cdna library  (TaKaRa)


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    Structured Review

    TaKaRa human kidney cdna library
    Human Kidney Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+kidney+cdna+library/Human+Kidney+QUICK-Clone+cDNA/us12521375-218-22-26
    Average 94 stars, based on 325 article reviews
    human kidney cdna library - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: SNF8, a member of the ESCRT-II complex, interacts with TRPC6 and enhances its channel activity
    Article Snippet: cDNA encoding residues 1 through 406 of TRPC6 (wild-type N-terminal domain) was used as bait and cloned in-frame with GAL4 DNA-binding domain in the vector pGBKT7-BD and transformed into yeast strain AH109. .. The bait strain was mated to Y187 yeast strain pre-transformed with a commercially available human kidney cDNA library cloned into pACT2-AD vector according to the manufacturer’s protocol (Clontech). ..

    Article Title: BICD2 phosphorylation regulates dynein function and centrosome separation in G2 and M
    Article Snippet: BICD2 fragments were eluted with 10 mM reduced l -glutathione (Merck Life Science). .. PLK1 PBD was kept bound to the agarose and stored at 4 °C for subsequent pull-down experiments. pGEX-Rab6A, containing the cDNA of human Rab6A, was a gift from Anna Akhmanova (Utrecht University) and is described in ref. . RanBP2 BBD (residues 2147–2287) was cloned from a human kidney cDNA library (Clontech) and inserted into the PGEX 4T1 vector. ..

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia.
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’- G G A A T T C G A T G C C C A G G G A A G A C A G-3 ́ and reverse primer 5’- C C G C T C G A G T T A G T C A A A G A G A C C A A A T C C-3 ́. ..

    Article Title: Functional Characterization of Glycine N -Methyltransferase and Its Interactive Protein DEPDC6/DEPTOR in Hepatocellular Carcinoma
    Article Snippet: Human GNMT cDNA was subcloned into the pGBKT7 vector (Clontech, Palo Alto, CA, USA). .. A human kidney cDNA library fused to the pACT2 vector (Clontech) was used as the prey. ..

    Article Title: SNF8, a member of the ESCRT-II complex, interacts with TRPC6 and enhances its channel activity
    Article Snippet: .. The Matchmaker Two-Hybrid System and S. cerevisiae Y187 pre-transformed with a human kidney cDNA library were purchased from Clontech (Palo Alto, CA). .. The dual luciferase assay kit and reporter vectors pGL4.30 and pGL4.74 were obtained from Promega.

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’-GGAATTCGATGCCCAGGGAAGACAG-3 ́ and reverse primer 5’-CCGCTCGAGTTAGTCAAAGAGACCAAATCC-3 ́. ..

    Article Title: Pharmaceutical composition for treating fatty liver disease
    Article Snippet: .. A cDNA encoding human ACC2 protein (27 amino acid residues to 2458 amino acid residues from the N-terminus) was cloned from a human kidney cDNA library (Clontech Laboratories, Inc.), a His-tag sequence was introduced at the 5′ end, and then inserted into pFastBac1 (Invitrogen). .. A recombinant baculovirus was produced in accordance with the protocol for a Bac-to-Bac baculovirus expression system (Invitrogen), and Sf-9 cells were infected therewith to express human ACC2 protein.

    Clone Assay:

    Article Title: SNF8, a member of the ESCRT-II complex, interacts with TRPC6 and enhances its channel activity
    Article Snippet: cDNA encoding residues 1 through 406 of TRPC6 (wild-type N-terminal domain) was used as bait and cloned in-frame with GAL4 DNA-binding domain in the vector pGBKT7-BD and transformed into yeast strain AH109. .. The bait strain was mated to Y187 yeast strain pre-transformed with a commercially available human kidney cDNA library cloned into pACT2-AD vector according to the manufacturer’s protocol (Clontech). ..

    Article Title: BICD2 phosphorylation regulates dynein function and centrosome separation in G2 and M
    Article Snippet: BICD2 fragments were eluted with 10 mM reduced l -glutathione (Merck Life Science). .. PLK1 PBD was kept bound to the agarose and stored at 4 °C for subsequent pull-down experiments. pGEX-Rab6A, containing the cDNA of human Rab6A, was a gift from Anna Akhmanova (Utrecht University) and is described in ref. . RanBP2 BBD (residues 2147–2287) was cloned from a human kidney cDNA library (Clontech) and inserted into the PGEX 4T1 vector. ..

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia.
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’- G G A A T T C G A T G C C C A G G G A A G A C A G-3 ́ and reverse primer 5’- C C G C T C G A G T T A G T C A A A G A G A C C A A A T C C-3 ́. ..

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’-GGAATTCGATGCCCAGGGAAGACAG-3 ́ and reverse primer 5’-CCGCTCGAGTTAGTCAAAGAGACCAAATCC-3 ́. ..

    Article Title: Pharmaceutical composition for treating fatty liver disease
    Article Snippet: .. A cDNA encoding human ACC2 protein (27 amino acid residues to 2458 amino acid residues from the N-terminus) was cloned from a human kidney cDNA library (Clontech Laboratories, Inc.), a His-tag sequence was introduced at the 5′ end, and then inserted into pFastBac1 (Invitrogen). .. A recombinant baculovirus was produced in accordance with the protocol for a Bac-to-Bac baculovirus expression system (Invitrogen), and Sf-9 cells were infected therewith to express human ACC2 protein.

    Plasmid Preparation:

    Article Title: BICD2 phosphorylation regulates dynein function and centrosome separation in G2 and M
    Article Snippet: BICD2 fragments were eluted with 10 mM reduced l -glutathione (Merck Life Science). .. PLK1 PBD was kept bound to the agarose and stored at 4 °C for subsequent pull-down experiments. pGEX-Rab6A, containing the cDNA of human Rab6A, was a gift from Anna Akhmanova (Utrecht University) and is described in ref. . RanBP2 BBD (residues 2147–2287) was cloned from a human kidney cDNA library (Clontech) and inserted into the PGEX 4T1 vector. ..

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia.
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’- G G A A T T C G A T G C C C A G G G A A G A C A G-3 ́ and reverse primer 5’- C C G C T C G A G T T A G T C A A A G A G A C C A A A T C C-3 ́. ..

    Article Title: Functional Characterization of Glycine N -Methyltransferase and Its Interactive Protein DEPDC6/DEPTOR in Hepatocellular Carcinoma
    Article Snippet: Human GNMT cDNA was subcloned into the pGBKT7 vector (Clontech, Palo Alto, CA, USA). .. A human kidney cDNA library fused to the pACT2 vector (Clontech) was used as the prey. ..

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’-GGAATTCGATGCCCAGGGAAGACAG-3 ́ and reverse primer 5’-CCGCTCGAGTTAGTCAAAGAGACCAAATCC-3 ́. ..

    Polymerase Chain Reaction:

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia.
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’- G G A A T T C G A T G C C C A G G G A A G A C A G-3 ́ and reverse primer 5’- C C G C T C G A G T T A G T C A A A G A G A C C A A A T C C-3 ́. ..

    Article Title: Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia
    Article Snippet: .. P0 was cloned into pGEX-4 T-3 plasmid by PCR from a human kidney cDNA library (BD Biosciences Clonetech) using forward primer 5’-GGAATTCGATGCCCAGGGAAGACAG-3 ́ and reverse primer 5’-CCGCTCGAGTTAGTCAAAGAGACCAAATCC-3 ́. ..

    Sequencing:

    Article Title: Pharmaceutical composition for treating fatty liver disease
    Article Snippet: .. A cDNA encoding human ACC2 protein (27 amino acid residues to 2458 amino acid residues from the N-terminus) was cloned from a human kidney cDNA library (Clontech Laboratories, Inc.), a His-tag sequence was introduced at the 5′ end, and then inserted into pFastBac1 (Invitrogen). .. A recombinant baculovirus was produced in accordance with the protocol for a Bac-to-Bac baculovirus expression system (Invitrogen), and Sf-9 cells were infected therewith to express human ACC2 protein.



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    Y2H cDNA library construction and screening procedure. A) The conventional procedure for cDNA library generation. B) Using the cDNA library in Y2H screens to identify protein–protein interactions. C) Using the cDNA library to screen for rescue genes involved in DNA repair. D) Screening randomly selected plasmids from the cDNA library to screen random samples from the library “Created with Biorender.com .”.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: Y2H cDNA library construction and screening procedure. A) The conventional procedure for cDNA library generation. B) Using the cDNA library in Y2H screens to identify protein–protein interactions. C) Using the cDNA library to screen for rescue genes involved in DNA repair. D) Screening randomly selected plasmids from the cDNA library to screen random samples from the library “Created with Biorender.com .”.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: cDNA Library Assay, Protein-Protein interactions

    The 5′ end truncated cDNAs identified, bp; base pairs.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: The 5′ end truncated cDNAs identified, bp; base pairs.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: Variant Assay, Binding Assay, RNA Binding Assay

    mRNA priming in cDNA libraries. A) Normal priming through reverse transcriptase elongation of cDNA after oligo (dT) binding to true poly-A tails. B) Internal priming that leads to the generation of truncated transcripts due to internal A-rich regions and reverse transcriptase stalling. “Created with Biorender.com .”.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: mRNA priming in cDNA libraries. A) Normal priming through reverse transcriptase elongation of cDNA after oligo (dT) binding to true poly-A tails. B) Internal priming that leads to the generation of truncated transcripts due to internal A-rich regions and reverse transcriptase stalling. “Created with Biorender.com .”.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: Reverse Transcription, Binding Assay

    Mitochondrial hits status and their quantification.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: Mitochondrial hits status and their quantification.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: